Composite

Part:BBa_K5250020:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter + T7-like RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal SapI site found at 2519
    Illegal SapI.rc site found at 1714


Design Notes

The sequences were codon optimized.


Source

The sequence for the T7-like RNAP is taken from https://doi.org/10.3390/ijms24087175 The rest of the sequences are taken from the iGEM Parts Registry.

References